Plasmid

Part:BBa_K4129032

Designed by: Christian Wistrm   Group: iGEM22_DTU-Denmark   (2022-10-08)


cas9 and gRNA #1 for insertion site #3 in aspergillus niger

Will make double stranded break at insertion site #3 in aspergillus niger

BioBricks in this part:
BBa_K4129023 - protospacer
BBa_K3385003 - gRNA backbone
BBa_K3385002 - tRNA glycin, A. niger
BBa_K3385007 - cas9
BBa_K678042 - hygR
BBa_K3385004 - U3 promoter
BBa_K3385005 - U3 terminator

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3078
    Illegal EcoRI site found at 5355
    Illegal EcoRI site found at 7609
    Illegal EcoRI site found at 7780
    Illegal EcoRI site found at 10067
    Illegal EcoRI site found at 10330
    Illegal EcoRI site found at 11975
    Illegal XbaI site found at 1257
    Illegal XbaI site found at 2023
    Illegal XbaI site found at 2505
    Illegal XbaI site found at 3273
    Illegal XbaI site found at 5160
    Illegal XbaI site found at 5928
    Illegal XbaI site found at 6410
    Illegal SpeI site found at 1440
    Illegal SpeI site found at 1451
    Illegal SpeI site found at 1815
    Illegal SpeI site found at 1947
    Illegal SpeI site found at 2071
    Illegal SpeI site found at 2178
    Illegal SpeI site found at 2189
    Illegal PstI site found at 4222
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3078
    Illegal EcoRI site found at 5355
    Illegal EcoRI site found at 7609
    Illegal EcoRI site found at 7780
    Illegal EcoRI site found at 10067
    Illegal EcoRI site found at 10330
    Illegal EcoRI site found at 11975
    Illegal NheI site found at 7453
    Illegal SpeI site found at 1440
    Illegal SpeI site found at 1451
    Illegal SpeI site found at 1815
    Illegal SpeI site found at 1947
    Illegal SpeI site found at 2071
    Illegal SpeI site found at 2178
    Illegal SpeI site found at 2189
    Illegal PstI site found at 4222
    Illegal NotI site found at 1296
    Illegal NotI site found at 14975
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3078
    Illegal EcoRI site found at 5355
    Illegal EcoRI site found at 7609
    Illegal EcoRI site found at 7780
    Illegal EcoRI site found at 10067
    Illegal EcoRI site found at 10330
    Illegal EcoRI site found at 11975
    Illegal BglII site found at 7082
    Illegal BamHI site found at 11141
    Illegal BamHI site found at 13489
    Illegal XhoI site found at 3619
    Illegal XhoI site found at 4336
    Illegal XhoI site found at 4814
    Illegal XhoI site found at 12500
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3078
    Illegal EcoRI site found at 5355
    Illegal EcoRI site found at 7609
    Illegal EcoRI site found at 7780
    Illegal EcoRI site found at 10067
    Illegal EcoRI site found at 10330
    Illegal EcoRI site found at 11975
    Illegal XbaI site found at 1257
    Illegal XbaI site found at 2023
    Illegal XbaI site found at 2505
    Illegal XbaI site found at 3273
    Illegal XbaI site found at 5160
    Illegal XbaI site found at 5928
    Illegal XbaI site found at 6410
    Illegal SpeI site found at 1440
    Illegal SpeI site found at 1451
    Illegal SpeI site found at 1815
    Illegal SpeI site found at 1947
    Illegal SpeI site found at 2071
    Illegal SpeI site found at 2178
    Illegal SpeI site found at 2189
    Illegal PstI site found at 4222
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3078
    Illegal EcoRI site found at 5355
    Illegal EcoRI site found at 7609
    Illegal EcoRI site found at 7780
    Illegal EcoRI site found at 10067
    Illegal EcoRI site found at 10330
    Illegal EcoRI site found at 11975
    Illegal XbaI site found at 1257
    Illegal XbaI site found at 2023
    Illegal XbaI site found at 2505
    Illegal XbaI site found at 3273
    Illegal XbaI site found at 5160
    Illegal XbaI site found at 5928
    Illegal XbaI site found at 6410
    Illegal SpeI site found at 1440
    Illegal SpeI site found at 1451
    Illegal SpeI site found at 1815
    Illegal SpeI site found at 1947
    Illegal SpeI site found at 2071
    Illegal SpeI site found at 2178
    Illegal SpeI site found at 2189
    Illegal PstI site found at 4222
    Illegal NgoMIV site found at 4356
    Illegal NgoMIV site found at 9162
    Illegal AgeI site found at 7324
    Illegal AgeI site found at 11448
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6949
    Illegal BsaI.rc site found at 1484
    Illegal BsaI.rc site found at 7282
    Illegal BsaI.rc site found at 9125
    Illegal BsaI.rc site found at 12146
    Illegal SapI site found at 9109
    Illegal SapI site found at 9505
    Illegal SapI site found at 13230
    Illegal SapI.rc site found at 7666


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